Vanessa Hudgens as Sunny Starscout (voice)

Kimiko Glenn as Izzy Moonbow (voice)

James Marsden as Hitch Trailblazer (voice)

Sofia Carson as Pipp Petals (voice)

Liza Koshy as Zipp Storm (voice)

Ken Jeong as Sprout (voice)

Elizabeth Perkins as Phyllis (voice)

Jane Krakowski as Queen Haven (voice)

Phil LaMarr as Alphabittle (voice)

Michael McKean as Argyle (voice)

Tabitha St. Germain as Rarity (voice)

Tara Strong as Twilight Sparkle (voice)

Andrea Libman as Fluttershy / Pinkie Pie (voice)

Ashleigh Ball as Applejack / Rainbow Dash

Arturo A. Hernandez as Toots / Thunder / Jasper / Pegasus Guard (voice)

Brooke Goldner as Sweets (voice)

Gillian M. Berrow as Zoom (voice)

Alan Schmuckler as Sprout (singing voice)

Heather Langenkamp as Dazzle Feather / Mayflower / Confused Mom (voice)

Will Friedle as Skye Silver / Glitter Cupcake / Comet Tail (voice)

Robert Cullen as Sparkle Chaser / Rob the Pony / Wings (voice)

Laura Bailey as Kid Earth Pony 1 / Pippsqueak 1 (voice)

Kari Wahlgren as Pegasus Fan (voice)

Tony Matthews as Balloon Pony / Unicorn Father / Worker Pony 1 (voice)

Lisa Linder as Beatnik Unicorn / Pegasus Concert Goer / Tram Driver (voice)

Thomas Rickert as Announcer Pony (voice)

Inbal Elazari as Bunnies (voice)

Inbal Elazari as Unicorn Kid (voice)