Annabelle Wallis as Madison Mitchell

Maddie Hasson as Sydney Lake

George Young as Detective Kekoa Shaw

Michole Briana White as Detective Regina Moss

Susanna Thompson as Jeanne

Ingrid Bisu as Winnie

Jean Louisa Kelly as Jane Doe

Jake Abel as Derek Mitchell

Jacqueline McKenzie as Dr. Florence Weaver

Christian Clemenson as Dr. Victor Fields

Amir Aboulela as Dr. Gregory

Mercedes Colon as Mendez

Ruben Pla as Officer Rubin

Jon Lee Brody as Officer Lee

Paula Marshall as Beverly Woods

Zoë Bell as Scorpion

Shaunte Lynette Johnson as Beta

Natalia Safran as Nurse Annie

Mike Mendez as Hospital Security Guard #1

Randy Haynie as Hospital Security Guard #2

Cord Walker as Hospital Security Guard #3

Jesus Trujillo as Research Doctor

Lisa Catara as Research Orderly

Danielle Nottingham as News Anchor

Ray Chase as Gabriel (voice)

Marina Mazepa as Gabriel

Mckenna Grace as Young Madison

Madison Wolfe as Young Serena

Andy Bean as Frank

Patricia Velásquez as Nurse Velasquez

Patrick Cox as Mr. Stanley (uncredited)

Gregory Garcia as Seattle Police Officer (uncredited)

Emir García as Mustang Driver (uncredited)

Renee Harbek as Caroline (uncredited)

Janet Hsieh as Nurse J. De Marco (uncredited)

Paul Mabon as Orderly (uncredited)

Gino Montesinos as Seattle Police Officer (uncredited)

Josh Rutgers as Referee (uncredited)

Halston Van Atta as Child Madison (uncredited)

Louise Van Veenendaal as Prisoner (uncredited)

Kalina Vanska as Seattle Police Officer (uncredited)

Christina Veronica as Prisoner (uncredited)

Rachel Winfree as Mrs. Ryerson (uncredited)