Sean Connery as James Bond

Ursula Andress as Honey Ryder

Joseph Wiseman as Dr. No

Jack Lord as Felix Leiter

Anthony Dawson as Professor R. J. Dent

Zena Marshall as Miss Taro

John Kitzmiller as Quarrel

Eunice Gayson as Sylvia Trench

Bernard Lee as M

Lois Maxwell as Miss Moneypenny

Peter Burton as Major Boothroyd

Yvonne Shima as Sister Lily

Michel Mok as Sister Rose

Marguerite LeWars as Annabel Chung - Photographer

William Foster-Davis as Superintendent Duff

Dolores Keator as Mary Trueblood

Reginald Carter as Mr. Jones

Louis Blaazer as Pleydell-Smith

Colonel Burton as General Potter

John Hatton as Signals Foreman (uncredited)

Byron Lee as Bandleader (uncredited)

Timothy Napier Moxon as Professor John Strangways (uncredited)

Malou Pantera as Hotel Receptionist (uncredited)

Lester Prendergast as Puss Feller (uncredited)

Diana Coupland as Honey Ryder - Singing Voice (voice) (uncredited)

Nikki Van der Zyl as Honey Ryder / Sylvia Trench / Various (voice) (uncredited)

Adrian Robinson as Hearse Driver

Bob Simmons as James Bond in Gunbarrel Sequence (uncredited)