Adam Devine as Ed (voice)

Rachel Bloom as Op (voice)

Zazie Beetz as Dottie (voice)

Ken Jeong as Clarance (voice)

Jim Jefferies as Burnie (voice)

Catherine O'Hara as Alma (voice)

Reggie Watts as Hoss / Ernest Shackleton (voice)

Henry Winkler as Jepson (voice)

Alex Borstein as Mali (voice)

Benedict Wong as Dr. Chung (voice)

Julia Chan as Mrs. Sun / Little Girl (voice)

Tom Hollander as Charles Darwin (voice)

Richard Kind as Wally (voice)

Steve Aoki as Vinny (voice)

Nick Frost as Captain (voice)

Jon Lovitz as Conch (voice)

Sydney Liu as Bo (voice)

Maria Bamford as Bo's Mom (voice)

Raymond S. Persi as That Guy / Booby (voice)

Terry Gross as Bomb (voice)

David Silverman as Cyclops / Donut Store Clerk (voice)

Kelly Hu as News Reporter (voice)

Tom Kenny as Mozart / Sled Dogs (voice)

Jason Hightower as Narrator / Sled Dogs (voice)

Kevin Michael Richardson as Sled Dogs (voice)

Brenden P. Hill as Baseball Announcer (voice)

Wilhelm Boyens as Additional Voices