Tomokazu Sugita as Sakata Gintoki (voice)

Daisuke Sakaguchi as Shimura Shinpachi (voice)

Rie Kugimiya as Kagura (voice)

Akira Ishida as Katsura Kotarou (voice)

Takehito Koyasu as Takasugi Shinsuke (voice)

Yutaka Aoyama as Okada Nizou (voice)

Takeharu Oonishi as Murata Tetsuya (voice)

Keiko Nemoto as Murata Tetsuko (voice)

Chafurin as Henpeita Takeshi (voice)

Risa Hayamizu as Kijima Matako (voice)

Kiyonobu Suzuki as Murata Jintetsu (voice)

Satsuki Yukino as Shimura Tae (voice)

Mikako Takahashi as Sadaharu (voice)

Susumu Chiba as Kondou Isao (voice)

Kazuya Nakai as Hijikata Toushirou (voice)

Kenichi Suzumura as Okita Sougo (voice)

Tetsuharu Ota as Yamazaki Sagaru (voice)

Takumi Yamazaki as Kawakami Bansai (voice)

Hochu Otsuka as Abuto (voice)

Satoshi Hino as Kamui (voice)

Sumi Shimamoto as Okita Mitsuba (voice)

Kujira as Terada Ayano (voice)

Yuu Sugimoto as Catherine (voice)

Yu Kobayashi as Sarutobi Ayame (voice)

Fumiko Orikasa as Yagyuu Kyuubee (voice)

Koji Yusa as Toujou Ayumu (voice)

Fumihiko Tachiki as Hasegawa Taizou (voice)

Shin-ichiro Miki as Sakamoto Tatsuma (voice)

Norio Wakamoto as Matsudaira Katakuriko (voice)

Kouichi Sakaguchi as Prince Hata (voice)

Shinji Takamatsu as Elizabeth (voice)

Koichi Yamadera as Yoshida Shouyou (voice)

Shigeo Kiyama as Additional Voices

Tsutomu Densaka as Additional Voices

Masahiro Okazaki as Additional Voices