Tomokazu Sugita as Gintoki Sakata (voice)

Daisuke Sakaguchi as Shinpachi Shimura (voice)

Rie Kugimiya as Kagura (voice)

Akira Ishida as Kotarou Katsura (voice)

Susumu Chiba as Isao Kondo (voice)

Kazuya Nakai as Toshiro Hijitaka (voice)

Kenichi Suzumura as Shogo Okita (voice)

Satsuki Yukino as Tae Shimura (voice)

Yu Kobayashi as Ayame Sarutobi (voice)

Fumiko Orikasa as Kyube Yakyu (voice)

Yuhko Kaida as Tsukuyo (voice)

Tsutomu Isobe as Enmi (voice)

Omi Minami as Tama (voice)

Yuu Sugimoto as Catherine (voice)

Akira Kamiya as Elizabeth (voice)

Fumihiko Tachiki as Taizou Hasegawa (voice)

Bin Shimada as Gengai Hiraga (voice)

Koichi Yamadera as Time Thief (voice)

Kouichi Sakaguchi as Musashi (voice)

Mikako Takahashi as Sadaharu (voice)

Shin-ichiro Miki as Tatsuma Sakamoto (voice)

Takehito Koyasu as Shinsuke Takasugi (voice)

Kujira as Ayano Terada (voice)

Koji Yusa as Ayumu Toujou (voice)

Tetsuharu Ota as Sagaru Yamazaki (voice)

Hirokazu Miura as Actor (voice)

Hiroki Goto as Actor (voice)

Hironori Kondoh as Amanto (voice)

Eiichiro Tokumoto as Chinpira A (voice)

Ryo Kuratomi as Chinpira B (voice)