Tomokazu Sugita as Gintoki Sakata (voice)

Daisuke Sakaguchi as Shinpachi Shimura (voice)

Rie Kugimiya as Kagura (voice)

Kazuya Nakai as Toushirou Hijikata (voice)

Akira Ishida as Kotarou Katsura (voice)

Kenichi Suzumura as Sougo Okita (voice)

Takehito Koyasu as Shinsuke Takasugi (voice)

Susumu Chiba as Isao Kondou (voice)

Mikako Takahashi as Sadaharu (voice)

Koichi Yamadera as Utsuro / Shouyou Yoshida (voice)

Satoshi Hino as Kamui (voice)

Hochu Otsuka as Abuto (voice)

Yuu Sugimoto as Catherine (voice)

Fumihiko Tachiki as Taizou Hasegawa (voice)

Kouichi Sakaguchi as Prince Hata (voice)

Toshiyuki Morikawa as Zenzou Hattori (voice)

Bin Shimada as Gengai Hiraga (voice)

Aya Hirano as Nobume Imai (voice)

Risa Hayamizu as Matako Kijima (voice)

Norio Wakamoto as Katakuriko Matsudaira (voice)

Toshitaka Hirano as Ness (voice)

Kazuhiko Inoue as Oboro (voice)

Shin-ichiro Miki as Tatsuma Sakamoto (voice)

Yu Kobayashi as Ayame Sarutobi (voice)

Satsuki Yukino as Tae Shimura (voice)

Hideaki Sorachi as Hideaki Sorachi (voice)

Ryo Hirohashi as Princess Soyo (voice)

Chafurin as Henpeita Takechi (voice)

Omi Minami as Tama (voice)

Kujira as Ayano Terada (voice)

Yuhko Kaida as Tsukuyo (voice)

Show Hayami as Umibouzu (voice)

Fumiko Orikasa as Kyuubee Yagyuu (voice)

Tetsuharu Ota as Sagaru Yamazaki (voice)

Shinnosuke Ogami as Executive (voice)

Ryunosuke Watanuki as Executive (voice)

Masashi Sugawara as Hitsugi (voice)

Motoki Sakuma as Reporter (voice)

Kazuki Souya as Reporter (voice)

Fumiya Tanaka as Additional Voices

Yuki Okada as Additional Voices

Satoshi Niwa as Additional Voices

Souru Saito as Additional Voices

Serina Machiyama as Additional Voices

Chinatsu Hirose as Additional Voices

Gentoku Kumazawa as Additional Voices

Takushi Fujikawa as Additional Voices

Keisuke Hamaoka as Additional Voices

Yuto Yamamoto as Additional Voices

Ryunosuke Yamaguchi as Additional Voices

Kayu Machida as Additional Voices

Hiroki Matsuhisa as Additional Voices