Jensen Ackles as Batman / Bruce Wayne (voice)

Josh Duhamel as Harvey Dent (voice)

Naya Rivera as Catwoman / Selina Kyle (voice)

Billy Burke as Commissioner Gordon (voice)

Jack Quaid as Alberto (voice)

Titus Welliver as Carmine Falcone (voice)

Troy Baker as Joker (voice)

Frances Callier as Nurse Tamara (voice)

Greg Chun as Mickey Chen (voice)

David Dastmalchian as Calendar Man (voice)

Alastair Duncan as Alfred (voice)

Gary LeRoi Gray as Pearce (voice)

Amy Landecker as Barbara Gordon (voice)

Julie Nathanson as Gilda (voice)

Jim Pirri as Sal Maroni (voice)

Fred Tatasciore as Solomon Grundy (voice)