Jensen Ackles as Bruce Wayne / Batman (voice)

Josh Duhamel as Harvey Dent (voice)

Naya Rivera as Selina Kyle / Catwoman (voice)

Billy Burke as Commissioner James Gordon (voice)

Titus Welliver as Carmine 'The Roman' Falcone (voice)

Troy Baker as Joker (voice)

David Dastmalchian as Julian Day / Calendar Man (voice)

Amy Landecker as Barbara Gordon (voice)

Julie Nathanson as Gilda Dent (voice)

Alastair Duncan as Alfred Pennyworth (voice)

Jack Quaid as Alberto Falcone (voice)

Fred Tatasciore as Solomon Grundy (voice)

Frances Callier as Nurse Tamara (voice)

Greg Chun as Mickey Chen (voice)

Gary LeRoi Gray as Pearce (voice)

Jim Pirri as Sal Maroni (voice)