Jensen Ackles as Bruce Wayne / Batman (voice)

Josh Duhamel as Harvey Dent / Two-Face (voice)

Naya Rivera as Selina Kyle / Catwoman (voice)

Billy Burke as Commissioner James Gordon (voice)

Titus Welliver as Carmine 'The Roman' Falcone / Luigi Maroni (voice)

David Dastmalchian as Julian Day / Calendar Man / Oswald Cobblepot / Penguin (voice)

Julie Nathanson as Gilda Dent (voice)

Alastair Duncan as Alfred Pennyworth (voice)

Katee Sackhoff as Pamela Isley / Poison Ivy (voice)

Robin Atkin Downes as Jonathan Crane / Scarecrow / Thomas Wayne (voice)

John DiMaggio as Jervis Tetch / Mad Hatter (voice)

Laila Berzins as Sofia Falcone (voice)

Fred Tatasciore as Solomon Grundy / Vincent Falcone (voice)

Amy Landecker as Barbara Gordon / Carla Viti (voice)

Troy Baker as Joker (voice)

Alyssa Diaz as Renee Montoya (voice)

Gary LeRoi Gray as Pearce (voice)

Jim Pirri as Sal Maroni (voice)

Zach Callison as Young Bruce Wayne (voice)

Rick D. Wasserman as Bodyguard (voice)