Jensen Ackles as Batman / Bruce Wayne (voice)

Josh Duhamel as Harvey Dent / Two-Face (voice)

Naya Rivera as Catwoman / Selina Kyle (voice)

Billy Burke as Commissioner Gordon (voice)

Titus Welliver as Carmine Falcone / Luigi Maroni (voice)

Troy Baker as Joker (voice)

Laila Berzins as Sofia Falcone (voice)

Zach Callison as Young Bruce Wayne (voice)

David Dastmalchian as Calendar Man / Penguin (voice)

Alyssa Diaz as Renee Montoya (voice)

John DiMaggio as Mad Hatter (voice)

Robin Atkin Downes as Scarecrow / Thomas Wayne (voice)

Alastair Duncan as Alfred (voice)

Gary LeRoi Gray as Pearce (voice)

Amy Landecker as Barbara Gordon / Carla Viti (voice)

Julie Nathanson as Gilda (voice)

Jim Pirri as Sal Maroni (voice)

Katee Sackhoff as Poison Ivy (voice)

Fred Tatasciore as Solomon Grundy / Vincent Falcone (voice)

Rick D. Wasserman as Bodyguard (voice)