Brittany Howard as Thelma the Unicorn (voice)

Will Forte as Otis (voice)

Jemaine Clement as Vic Diamond (voice)

Edi Patterson as Megan (voice)

Maliaka Mitchell as Peggy Purvis (voice)

Ally Dixon as Nikki Narwhal (voice)

Fred Armisen as Danny Stallion (voice)

Zach Galifianakis as Crusty Trucker (voice)

Jon Heder as Reggie (voice)

Kristen Schaal as Goat Girl (voice)

Natalie Morales as Penelope Pizazz (voice)

Eduardo Franco as Goon #2 (voice)

Sherry Cola as Female Talent Judge (voice)

Geno Segers as Male Talent Judge (voice)

Betsy Sodaro as Cow (voice)

Shondrella Avery as Zirconia (voice)

Héctor Jiménez as Esteban (voice)

James Urbaniak as Confused Fan (voice)

Phil Hendrie as Bald Guy (voice)

Jared Hess as Gerald (voice)

Lynn Wang as Chicken Lynn (voice)

Daran Norris as Stable Hand (voice)

David Michie as Farmer Bob (voice)

Pam Coats as Chicken Pam (voice)

Michelle Jubilee Gonzalez as Goat Farmhand (voice)

Matthew Wolf as Duck Judge (voice)

Ashley London as Woman Fan (voice)

Scott Whyte as Announcer (voice)

Roger Craig Smith as Garyl (voice)

Kimberly Bailey as Cheryl (voice)

Juan Pacheco as Beryl (voice)

Eric Bauza as Fan Boy (voice)

Grey DeLisle as Bridget (voice)

Scott Menville as Raccoon (voice)

Krizia Bajos as Female News Anchor (voice)

John Eric Bentley as Rude Fan (voice)

Noelle Holsinger as Suzie (voice)

Birdie Hess as Fluffy Rose (voice)

Carter Hurst as Goth Kid (voice)

K.J. Howard as Hunky Fella (voice)

Blaze James Gorman as Unicorn Fan (voice)

Mary Deaton as Prom Girl (voice)