Alyson Stoner as Little Engine (voice)

Whoopi Goldberg as The Tower (voice)

Patrick Warburton as The Caboose (voice)

Jeff Bennett as Hudson, Red Engine (voice)

Jodi Benson as Jillian (voice)

Corbin Bleu as Lou (voice)

Jim Cummings as Rusty (voice)

Jamie Lee Curtis as Beverly (voice)

Chelsea Evered as Girl Train (voice)

Khamani Griffin as Marcus (voice)

Dominic Scott Kay as Richard (voice)

Mocean Melvin as Big Locomotive (voice)

Suzy Nakamura as Teacher (voice)

Ray Porter as Nightmare Train (voice)

Michael S. Garcia as Scott (voice as Michael Rodrigo)

Rodney Saulsberry as Freight Train, Bud (voice)

Charlie Schlatter as Major (voice)

Brenda Song as Shiny Passenger Train (voice)