Shameik Moore as Miles Morales (voice)

Jake Johnson as Peter B. Parker (voice)

Hailee Steinfeld as Gwen Stacy (voice)

Mahershala Ali as Uncle Aaron (voice)

Brian Tyree Henry as Jefferson Davis (voice)

Lily Tomlin as Aunt May (voice)

Luna Lauren Velez as Rio Morales (voice)

Zoë Kravitz as Mary Jane (voice)

John Mulaney as Spider-Ham (voice)

Kimiko Glenn as Peni Parker (voice)

Nicolas Cage as Spider-Man Noir (voice)

Kathryn Hahn as Doc Ock (voice)

Liev Schreiber as Wilson Fisk (voice)

Chris Pine as Peter Parker (voice)

Natalie Morales as Miss Calleros (voice)

Edwin H. Bravo as Brooklyn Visions Security Guard (voice)

Oscar Isaac as Interesting Person #1 (voice)

Greta Lee as Interesting Person #2 (voice)

Stan Lee as Stan (voice)

Jorma Taccone as Green Goblin / Last Dude (voice)

Joaquín Cosío as Scorpion (voice)

Marvin Jones III as Tombstone (voice)

Kim Yarbrough as Scientist in Cafeteria (voice)

Lake Bell as Vanessa Fisk (voice)

Jessica Mikayla Adams as Brooklyn Friend (voice)

Gredel Berrios Calladine as Brooklyn Friend (voice)

Sarah D. Cole as Brooklyn Friend (voice)

Kelby Joseph as Brooklyn Friend (voice)

Mimi Davila as Brooklyn Visions Student (voice)

Claudia Choi as Brooklyn Visions Teacher (voice)

Melanie Haynes as Brooklyn Visions Teacher (voice)

Joseph Izzo as Brooklyn Visions Teacher (voice)

Nick Jaine as Brooklyn Visions Teacher (voice)

Muneeb Rehman as Brooklyn Visions Teacher (voice)

Carlos Zaragoza as Brooklyn Visions Teacher (voice)

Post Malone as Brooklyn Bystander (voice)

David Applebee as Additional Voices (voice)

Juan Carlos Arvelo as Additional Voices (voice)

Adam Brown as Additional Voices (voice)

Jon Bruno as Additional Voices (voice)

Darcy Rose Byrnes as Additional Voices (voice)

Oscar Camacho as Additional Voices (voice)

June Christopher as Additional Voices (voice)

Alycia Cooper as Additional Voices (voice)

Michelle Jubilee Gonzalez as Additional Voices (voice)

Terrence Hardy Jr. as Additional Voices (voice)

Bridget Hoffman as Additional Voices (voice)

Rif Hutton as Additional Voices (voice)

Miguel Jiron as Additional Voices (voice)

Harrison Knight as Additional Voices (voice)

Lex Lang as Additional Voices (voice)

Donna Lynn Leavy as Additional Voices (voice)

Andrew Leviton as Additional Voices (voice)

Caitlin McKenna as Additional Voices (voice)

Scott Menville as Additional Voices (voice)

Christopher Miller as Additional Voices (voice)

Arthur Ortiz as Additional Voices (voice)

Juan Pacheco as Additional Voices (voice)

Devika Parikh as Additional Voices (voice)

Shakira Ja'nai Paye as Additional Voices (voice)

Courtney Peldon as Additional Voices (voice)

Chrystee Pharris as Additional Voices (voice)

Jacqueline Pinol as Additional Voices (voice)

Juan Pope as Additional Voices (voice)

Al Rodrigo as Additional Voices (voice)

Joseph Sanfelippo as Additional Voices (voice)

Justin Shenkarow as Additional Voices (voice)

Dennis Singletary as Additional Voices (voice)

Warren Sroka as Additional Voices (voice)

Melissa Sturm as Additional Voices (voice)

Holly Walker as Additional Voices (voice)

Jason Linere-White as Additional Voices (voice)

Ruth Zalduondo as Additional Voices (voice)