Dave Foley as Flik (voice)

Kevin Spacey as Hopper (voice)

Julia Louis-Dreyfus as Atta (voice)

Hayden Panettiere as Dot (voice)

Phyllis Diller as Queen (voice)

Richard Kind as Molt (voice)

David Hyde Pierce as Slim (voice)

Joe Ranft as Heimlich / Additional Voices (voice)

Denis Leary as Francis (voice)

Jonathan Harris as Manny (voice)

Madeline Kahn as Gypsy Moth (voice)

Bonnie Hunt as Rosie (voice)

Michael McShane as Tuck / Roll (voice)

John Ratzenberger as P.T. Flea (voice)

Brad Garrett as Dim (voice)

Roddy McDowall as Mr. Soil (voice)

Edie McClurg as Dr. Flora (voice)

Alex Rocco as Thorny (voice)

David Ossman as Cornelius (voice)

Carlos Alazraqui as Additional Voices (voice)

Jack Angel as Additional Voices (voice)

Bob Bergen as Additional Voices (voice)

Kimberly J. Brown as Additional Voices (voice)

Rodger Bumpass as Additional Voices (voice)

Anthony Burch as Additional Voices (voice)

Jennifer Darling as Additional Voices (voice)

Rachel Davey as Additional Voices (voice)

Debi Derryberry as Additional Voices (voice)

Paul Eiding as Additional Voices (voice)

Jessica Evans as Additional Voices (voice)

Bill Farmer as Additional Voices (voice)

Sam Gifaldi as Additional Voices (voice)

Brad Hall as Additional Voices (voice)

Jess Harnell as Additional Voices (voice)

Brenden Hickey as Additional Voices (voice)

Kate Charlotte Hodges as Additional Voices (voice)

Denise Johnson as Additional Voices (voice)

David L. Lander as Additional Voices (voice)

John Lasseter as Additional Voices (voice)

Sherry Lynn as Additional Voices (voice)

Mickie McGowan as Additional Voices (voice)

Courtland Mead as Additional Voices (voice)

Christina Milian as Additional Voices (voice)

Kelsey Mulrooney as Additional Voices (voice)

Ryan O'Donohue as Additional Voices (voice)

Jeff Pidgeon as Additional Voices (voice)

Phil Proctor as Additional Voices (voice)

Jan Rabson as Additional Voices (voice)

Jordan Ranft as Additional Voices (voice)

Brian M. Rosen as Additional Voices (voice)

Rebecca Schneider as Additional Voices (voice)

Francesca Marie Smith as Additional Voices (voice)

Andrew Stanton as Additional Voices (voice)

Hannah Swanson as Additional Voices (voice)

Russi Taylor as Additional Voices (voice)

Travis Tedford as Additional Voices (voice)

Ashley Tisdale as Additional Voices (voice)

Lee Unkrich as Additional Voices (voice)

Jordan Warkol as Additional Voices (voice)