Humphrey Bogart as Samuel Spade

Mary Astor as Brigid O'Shaughnessy

Gladys George as Iva Archer

Peter Lorre as Joel Cairo

Barton MacLane as Lt. of Detectives Dundy

Lee Patrick as Effie Perine

Sydney Greenstreet as Kasper Gutman

Ward Bond as Det. Tom Polhaus

Jerome Cowan as Miles Archer

Elisha Cook Jr. as Wilmer Cook

James Burke as Luke

Murray Alper as Frank Richman

John Hamilton as District Attorney Bryan

Charles Drake as Reporter (uncredited)

Chester Gan as Bit Part (uncredited)

Creighton Hale as Stenographer (uncredited)

Robert Homans as Policeman (uncredited)

William Hopper as Reporter (uncredited)

Walter Huston as Captain Jacoby (uncredited)

Hank Mann as Reporter (uncredited)

Jack Mower as Announcer (uncredited)

Emory Parnell as Ship's Mate (uncredited)