Freddie Prinze Jr. as Fred

Sarah Michelle Gellar as Daphne

Matthew Lillard as Shaggy

Linda Cardellini as Velma

Neil Fanning as Scooby Voice (voice)

Rowan Atkinson as Mondavarious

Isla Fisher as Mary Jane

Miguel A. Núñez Jr. as Voodoo Maestro

Steven Grives as N' Goo Tuana

Charles Stan Frazier as Sugar Ray

Craig Bullock as Sugar Ray

Matthew Murphy Karges as Sugar Ray

Mark McGrath as Sugar Ray

Rodney Sheppard as Sugar Ray

Sam Greco as Zarkos

Charlie Cousins as Velma's Friend

Kristian Schmid as Brad

Nicholas Hope as Old Man Smithers

Scott Innes as Scrappy Voice (voice)

J.P. Manoux as Scrappy Rex Voice (voice)

Chris Cruickshanks as Tiny Henchman

Alex Ruiz as Reporter #1

Sheryl Benko as Reporter #2

Rio Nugara as Island Emissary

David Vallon as Bartender Dead Mike's

Troy MacKinder as Guy in the Vat

Michala Banas as Carol

Holly Brisley as Training Video Woman

Robert Díaz as Training Video Guy #1

Remi Broadway as Training Video Guy #2

Martin Broome as Melvin Doo

Simone Dumbleton as Co-Ed

Jonathan Coffey as Fitzgibbon

Michael Caffery as Coast Guard #2

Kyas Sherriff as Airport Attendant

Celeste Gosnell as Airport Family

Bradley Gosnell as Airport Family

Cayley Gosnell as Airport Family

Audrey Gosnell as Airport Family

Kurt Duval as Spooky Hotel Bartender

Janis McGavin as Co-Ed Hottie

Emily Gosnell as Autograph Seeker

Marea Lambert Barker as Autograph Seeker

Kym Jackson as Autograph Seeker

Danielle Starkey as Autograph Seeker

Andrew Bryniarski as Cavern Henchman

Craig Behenna as Henchman #1

Keith S. Bullock as Henchman #2

Jess Harnell as Creature Voices (voice)

Frank Welker as Creature Voices (voice)

Pamela Anderson as Pamela Anderson (uncredited)