Seth Green as The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice)

Breckin Meyer as Superman / Mirror Master (voice)

Nathan Fillion as Green Lantern / Mr. Freeze (voice)

Alex Borstein as Wonder Woman / Giganta / Woman (voice)

Matthew Senreich as The Flash / Chillblaine / Brainiac / Wildcat (voice)

Alfred Molina as Lex Luthor / Firestorm / Mr. Banjo (voice)

Paul Reubens as The Riddler (voice)

Neil Patrick Harris as Two-Face / Black Manta (voice)

Kevin Shinick as Narrator / Captain Cold (voice)

Abraham Benrubi as Cyborg/ Kilowog / Appa Ali Apsa / Solomon Grundy (voice)

Zeb Wells as Sinestro / Swamp Thing (voice)

Megan Fox as Lois Lane (voice)

Aaron Paul as Glenn (voice)

Tara Strong as Harley Quinn / Selena Gomez (voice)

Tom Root as Icicle/B'dg (voice)