Frank Welker as Fred Jones / Scooby-Doo (voice)

Casey Kasem as Shaggy Rogers (voice)

Heather North as Daphne Blake (voice)

Nicole Jaffe as Velma Dinkley (voice)

Candi Milo as Charlene Otero / Museum Guide / Old Woman #1 (voice)

María Canals-Barrera as Sofia Otero / Old Woman #2 (voice)

Jesse Borrego as Luis Otero / Musician #2 / Suspicious Tourist (voice)

Brandon Gonzales as Jorge Otero (voice)

Castulo Guerra as Señor Fuente / Man #1 (voice)

Benito Martinez as El Curandero / Man #2 (voice)

Darlene Mendoza as Natalia Otero / Little Girl (voice)

Rita Moreno as Doña Dolores / Woman #3 (voice)

Allyse Rivera as Carolina Otero (voice)

Casey Sandino as Sebastian Otero / Little Boy (voice)

Eddie Santiago as Alejo Otero / Musician #1 (voice)

Rip Taylor as Mr. Smiley / Ghost of Señor Otero (voice)

Michael Forest as El Chuprecabra (voice)