Casey Kasem as Shaggy Rogers (voice)

Frank Welker as Fred Jones / Scooby-Doo / The Centaur (voice)

Grey DeLisle as Daphne Blake / Medusa (voice)

Mindy Cohn as Velma Dinkley (voice)

Mark Hamill as Barge Captain (voice)

Tara Strong as Alexandra Viggi (voice)

James Arnold Taylor as Sonny Les Matines / Guy L'Avorton (voice)

Nick Jameson as Lysander Demas / Scary Looking Man (voice)

Phil LaMarr as Toddy Stickfigger / Cabbie (voice)

Jess Harnell as Lue / Shane Flinty / Constable (voice)

Daran Norris as Ugo DiRinaldi / Shopkeeper (voice)

John DiMaggio as Dragon / Officious Dwarf / Judge (voice)

Rob Paulsen as Ripley / Pan Lute / Puny Guy (voice)

Jeff Bennett as Feta Cheese Vendor / Major Portsmouth / Shop Owner (voice)

Moira Quirk as Susie Smythe (voice)

Vanessa Marshall as Danica LeBlake (voice)

Tasia Valenza as Brooke Bjork (voice)