Haviland Stillwell as Avia Trotter (voice)

Laura Bailey as Headmistress Bloodgood / Lagoona Blue (voice)

Paula Rhodes as Iris Clops / Sirena Von Boo (voice)

Kate Higgins as Frankie Stein (voice)

America Young as Howleen Wolf / Toralei Stipe (voice)

Erin Fitzgerald as Abbey Bominable / Rochelle Goyle / Spectra Vondergeist (voice)

Geeg Friedman as Bonita Femur (voice)

Salli Saffioti as Clawdeen Wolf / Cleo de Nile (voice)

Evan Smith as Deuce Gorgon / Gil Webber (voice)

Debi Derryberry as Draculaura (voice)

Audu Paden as Ghoulia Yelps / The Creature / Manny Taur (voice)

Jonquil Goode as Gigi Grant / Twyla (voice)

Cam Clarke as Heath Burns / Mr. Rotter / Hoodude Voodoo (voice)

Dave B. Mitchell as Hexicah Steam (voice)

Cindy Robinson as Jackson Jekyll / Operetta (voice)

Stephanie Sheh as Jinafire Long (voice)

Luis Gonzalez as Mr. Where (voice)

Josey Montana McCoy as Neighthan Rot / Sparky / Victor Frankenstein (voice)

Julie Maddalena as Robecca Steam / Venus McFlytrap (voice)