Colin Clive as Henry Frankenstein

Mae Clarke as Elizabeth

John Boles as Victor Moritz

Boris Karloff as The Monster

Edward Van Sloan as Doctor Waldman

Frederick Kerr as Baron Frankenstein

Dwight Frye as Fritz

Lionel Belmore as The Burgomaster

Marilyn Harris as Little Maria

Ted Billings as Villager (uncredited)

Mae Bruce as Screaming Maid (uncredited)

Jack Curtis as Villager (uncredited)

Arletta Duncan as Bridesmaid (uncredited)

William Dyer as Gravedigger (uncredited)

Francis Ford as Hans (uncredited)

Soledad Jiménez as Mourner (uncredited)

Carmencita Johnson as Little Girl (uncredited)

Seessel Anne Johnson as Little Girl (uncredited)

Margaret Mann as Mourner (uncredited)

Michael Mark as Ludwig (uncredited)

Pauline Moore as Bridesmaid (uncredited)

Inez Palange as Villager (uncredited)

Paul Panzer as Mourner (uncredited)

Cecilia Parker as Maid (uncredited)

Rose Plumer as Villager (uncredited)

Cecil Reynolds as Waldman's Secretary (uncredited)

Ellinor Vanderveer as Medical Student (uncredited)

Robert Milasch as Villager (uncredited)