Will Arnett as Batman / Bruce Wayne (voice)

Michael Cera as Robin / Dick Grayson (voice)

Rosario Dawson as Batgirl / Barbara Gordon (voice)

Ralph Fiennes as Alfred Pennyworth (voice)

Zach Galifianakis as Joker (voice)

Jenny Slate as Harley Quinn (voice)

Jason Mantzoukas as Scarecrow (voice)

Conan O'Brien as The Riddler (voice)

Doug Benson as Bane (voice)

Billy Dee Williams as Two-Face (voice)

Zoë Kravitz as Catwoman (voice)

Kate Micucci as Clayface (voice)

Riki Lindhome as Poison Ivy (voice)

Eddie Izzard as Voldemort (voice)

Seth Green as King Kong (voice)

Jemaine Clement as Sauron (voice)

Ellie Kemper as Phyllis (voice)

Channing Tatum as Superman (voice)

Jonah Hill as Green Lantern (voice)

Adam Devine as The Flash (voice)

Héctor Elizondo as Jim Gordon (voice)

Mariah Carey as Mayor McCaskill (voice)

Lauren White as Chief O'Hara / Medusa / Additional Voices (voice)

David Burrows as Anchorman Phil (voice)

Laura Kightlinger as Reporter Pippa (voice)

Brent Musburger as Reporter #1 (voice)

Ralph Garman as Reporter #2 (voice)

Chris Hardwick as Reporter #3 (voice)

Todd Hansen as Captain Dale (voice)

Chris McKay as Pilot Bill / Additional Voices (voice)

Susan Bennett as 'Puter (voice)

Abel Arnett as Additional Voices (voice)

Archie Arnett as Additional Voices (voice)

Marco Campagno as Additional Voices (voice)

Garret Elkins as Additional Voices (voice)

Ryan Halprin as Additional Voices (voice)

Alison Ingram as Additional Voices (voice)

Holly Isaacson as Additional Voices (voice)

Kelly Lafferty as Additional Voices (voice)

Doug Nicholas as Additional Voices (voice)

Samantha Nisenboim as Additional Voices (voice)

Chris Paluszek as Additional Voices (voice)

Wendell Stephens as Additional Voices (voice)

Vanara Taing as Additional Voices (voice)

Matt Town as Additional Voices (voice)

Bram Tulloch as Additional Voices (voice)

John Venzon as Additional Voices (voice)

Matt Villa as Additional Voices (voice)

Charlie Bean as Additional Voices (voice)