Michael Sinterniklaas as Leonardo (voice)

Wayne Grayson as Michelangelo (voice)

Sam Riegel as Donatello (voice)

Gregory Abbey as Raphael (voice)

Darren Dunstan as Splinter (voice)

Marc Thompson as Casey Jones (voice)

Veronica Taylor as April O'Neil (voice)

Scott Rayow as The Shredder (voice)

Greg Carey as Hun (voice)

Karen Neil as Karai (voice)

Dan Green as 1988 Leonardo (voice)

Johnny Castro as 1988 Michelangelo / Rocksteady (voice)

Anthony Salerno as 1988 Donatello (voice)

Sebastian Arcelus as 1988 Raphael (voice)

David Wills as 1988 Splinter / 1984 Shredder (voice)

Rebecca Soler as 1988 April O'Neil (voice)

Load Williams as 1988 Shredder (voice)

Bradford Scobie as Krang / Bebop / 1984 Michelangelo (voice)

Jason Griffith as 1984 Leonardo / Additional Voices (voice)

Sean Schemmel as 1984 Raphael / Additional Voices (voice)

Clay Adams as 1984 Donatello (voice)

Bruce Falk as Additional Voices (voice)

Matthew Piazzi as Additional Voices (voice)

Kevin Eastman as Kevin Eastman (voice) (uncredited)

Peter Laird as Peter Laird (voice) (uncredited)