Thomas Middleditch as Lester (voice)

James Marsden as Hank (voice)

Rosario Dawson as Jolene (voice)

Alfred Molina as Baron Blackout (voice)

Will Sasso as The Gluttonator / Union Boss (voice)

Nathan Fillion as Captain Superior (voice)

Rob Riggle as Biff (voice)

Jane Krakowski as Jane (voice)

Craig Robinson as Stew (voice)

Bobcat Goldthwait as Jackalope (voice)

Brian Drummond as Old Doug (voice)

Bobby Henwood as Yakov (voice)

Ashleigh Ball as Terri Murphy / Pep Girl Twin (Iliad) (voice)

Kazumi Evans as Pep Girl Twin (Odyssey) (voice)

Kelly Sheridan as Additional Voices

Donavon Stinson as Nick of Time / Bloop (voice)

Ian Hanlin as Slap Happy / Bleep (voice)

Andy Toth as Chet (voice)

Donny Lucas as Brutus (voice)

Felix Wood as Young Lester (voice)

Jimi Cuell as Orientation Computer (voice)