Sidney Toler as Charlie Chan

Victor Sen Yung as Jimmy Chan (as Sen Yung)

C. Henry Gordon as Dr. Cream

Marc Lawrence as Steve McBirney

Eddie Marr as Henchman Grenock

Charles Wagenheim as Willie Fern, museum watchman

Joan Valerie as Lily Latimer

Marguerite Chapman as Mary Bolton

Hilda Vaughn as Mrs. Joe Rocke

Harold Goodwin as Edwards, radio sound man

Ted Osborne as Tom Agnew (as Ted Osborn)

Archie Twitchell as Carter Lane

Michael Visaroff as Dr. Otto Von Brom

Joe King as Inspector O'Matthews

Charles Trowbridge as Judge

Emmett Vogan as Prosecuting Attorney

Stanley Blystone as Courtroom Officer

Jimmy Conlin as Tour Bus Barker

Brick Sullivan as Detective Pete

Walter Bacon as Sidewalk Passerby (extra)

David Newell as Attorney at Trial (extra)