Natalie Lander as Krissy Holdout (voice)

Kate Micucci as Velma Dinkley (voice)

Matthew Lillard as Shaggy Rogers (voice)

Grey DeLisle as Daphne Blake (voice)

Iqbal Theba as Dr. Najib (voice)

Frank Welker as Scooby-Doo / Fred Jones (voice)

Kate Higgins as Brenda (voice)

Hynden Walch as Mitzi Capaletto (voice)

Jeff Bennett as Deputy / Museum Guide (voice)

Kevin Michael Richardson as Sheriff (voice)

Tom Kenny as Rob Holdout (voice)

Jack McBrayer as Police Officer (voice)

Fred Tatasciore as Dwight Monkfish (voice)

Josh Keaton as Tommie / Chad Holdout (voice)