Frank Welker as Scooby-Doo / Fred Jones (voice)

Grey DeLisle as Daphne Blake / Black Canary (voice)

Matthew Lillard as Shaggy Rogers (voice)

Kate Micucci as Velma Dinkley (voice)

Diedrich Bader as Batman (voice)

Jeff Bennett as Joker (voice)

Jeffrey Combs as The Question (voice)

John DiMaggio as Aquaman / Crimson Cloak (voice)

Nika Futterman as Catwoman (voice)

Nicholas Guest as Martian Manhunter (voice)

John Michael Higgins as The Riddler (voice)

Tom Kenny as Penguin / Plastic Man (voice)

Kevin Michael Richardson as Clayface / Detective Chimp (voice)

Tara Strong as Harley Quinn / Poison Ivy (voice)

Fred Tatasciore as Harvey Bullock (voice)