Larry the Cable Guy as Mater (voice)

Owen Wilson as Lightning McQueen (voice)

Michael Caine as Finn McMissile (voice)

Emily Mortimer as Holley Shiftwell (voice)

Eddie Izzard as Sir Miles Axlerod (voice)

John Turturro as Francesco Bernoulli (voice)

Brent Musburger as Brent Mustangburger (voice)

Joe Mantegna as Grem (voice)

Thomas Kretschmann as Professor Z (voice)

Peter Jacobson as Acer (voice)

Bonnie Hunt as Sally (voice)

Darrell Waltrip as Darrell Cartrip (voice)

Franco Nero as Uncle Topolino (voice)

David Hobbs as David Hobbscap (voice)

Patrick Walker as Mel Dorado (voice)

Tony Shalhoub as Luigi (voice)

Jeff Garlin as Otis (voice)

Michel Michelis as Tomber (voice)

Jason Isaacs as Siddeley / Leland Turbo (voice)

Lloyd Sherr as Fillmore / Combat Ship (voice)

Bruce Campbell as Rod "Torque" Redline (voice)

Teresa Gallagher as Mater's Computer (voice)

Jenifer Lewis as Flo (voice)

Stanley Townsend as Victor Hugo / Vladimir Trunkov / Ivan the Tow Truck (voice)

Velibor Topic as Alexander Hugo (voice)

Sig Hansen as Crabby (voice)

Guido Quaroni as Guido (voice)

Vanessa Redgrave as The Queen / Mama Topolino (voice)

John Mainieri as J. Curby Gremlin (voice)

Bradford Lewis as Tubbs Pacer (voice)

Cheech Marin as Ramone (voice)

Jeff Gordon as Jeff Gorvette (voice)

Lewis Hamilton as Lewis Hamilton (voice)

Paul Dooley as Sarge (voice)

Edie McClurg as Minny (voice)

Richard Kind as Van (voice)

Katherine Helmond as Lizzie (voice)

John Ratzenberger as Mack (voice)

Michael Wallis as Sheriff (voice)

Catie Bolt as Additional Voices (voice)

Gillian Bolt as Additional Voices (voice)

Jess Fulton as Additional Voices (voice)

Sonoko Konishi as Additional Voices (voice)

John Lasseter as Additional Voices (voice)

Daisuke "Dice" Tsutsumi as Additional Voices (voice)