Chris Pratt as Mario (voice)

Anya Taylor-Joy as Princess Peach (voice)

Charlie Day as Luigi (voice)

Jack Black as Bowser (voice)

Keegan-Michael Key as Toad (voice)

Seth Rogen as Donkey Kong (voice)

Fred Armisen as Cranky Kong (voice)

Sebastian Maniscalco as Spike (voice)

Charles Martinet as Mario's Dad / Giuseppe (voice)

Kevin Michael Richardson as Kamek (voice)

Khary Payton as Penguin King (voice)

Rino Romano as Uncle Tony (voice)

John DiMaggio as Uncle Arthur (voice)

Jessica DiCicco as Mario's Mom (voice)

Eric Bauza as Toad General (voice)

Juliet Jelenic as Lumalee (voice)

Scott Menville as Koopa General (voice)

Carlos Alazraqui as Additional Voices (voice)

Jason Broad as Additional Voices (voice)

Ashly Burch as Additional Voices (voice)

Rachel Butera as Additional Voices (voice)

Cathy Cavadini as Additional Voices (voice)

Will Collyer as Additional Voices (voice)

Django Craig as Additional Voices (voice)

Willow Geer as Additional Voices (voice)

Aaron Hendry as Additional Voices (voice)

Andy Hirsch as Additional Voices (voice)

Barbara Harris as Additional Voices (voice)

Phil LaMarr as Additional Voices (voice)

Jeremy Maxwell as Additional Voices (voice)

Daniel Mora as Additional Voices (voice)

Eric E. Osmond as Additional Voices (voice)

Noreen Reardon as Additional Voices (voice)

Lee Shorten as Additional Voices (voice)

Cree Summer as Additional Voices (voice)

Nisa Ward as Additional Voices (voice)

Nora Wyman as Additional Voices (voice)