Shameik Moore as Miles Morales (voice)

Hailee Steinfeld as Gwen Stacy (voice)

Brian Tyree Henry as Jeff Morales (voice)

Luna Lauren Velez as Rio Morales (voice)

Jake Johnson as Peter B. Parker (voice)

Oscar Isaac as Miguel O'Hara (voice)

Jason Schwartzman as Spot (voice)

Issa Rae as Jessica Drew (voice)

Daniel Kaluuya as Hobie Brown (voice)

Karan Soni as Pavitr Prabhakar (voice)

Shea Whigham as George Stacy (voice)

Greta Lee as LYLA (voice)

Mahershala Ali as Uncle Aaron (voice)

Amandla Stenberg as Margo Kess: Spider-Byte (voice)

Jharrel Jerome as Miles G. Morales (voice)

Andy Samberg as Ben Reilly (voice)

Jack Quaid as Peter Parker (voice)

Rachel Dratch as Ms. Weber (voice)

Ziggy Marley as Lenny (voice)

Jorma Taccone as Adriano Tumino / '67 (voice)

J.K. Simmons as J. Jonah Jameson (voice)

Donald Glover as Aaron Davis

Elizabeth Perkins as May / Quippy Spider-Person (voice)

Kathryn Hahn as Doc Ock (voice)

Ayo Edebiri as Glory (voice)

Nicole Delaney as MJ (voice)

Antonina Lentini as Betty (voice)

Atsuko Okatsuka as Yuri (voice)

Peter Sohn as Ganke (voice)

Melissa Sturm as Mary Jane (voice)

Lorraine Velez as Maria (voice)

Nic Novicki as Lego Spider-Man (voice)

Taran Killam as Web-Slinger (voice)

Metro Boomin as Metro Spider-Man (voice)

Josh Keaton as Spectacular Spider-Man (voice)

Sofia Barclay as Malala Windsor: Spider-UK (voice)

Danielle Perez as Charlotte Webber: Sun-Spider (voice)

Yuri Lowenthal as Insomniac Spider-Man (voice)

Rita Rani Ahuja as Additional Voices (voice)

Ismail Bashey as Additional Voices (voice)

Oscar Camacho as Additional Voices (voice)

Freddy Ferrari as Additional Voices (voice)

Kerry Gutierrez as Additional Voices (voice)

Kamal Khan as Additional Voices (voice)

Angelo Sekou Kouyate as Additional Voices (voice)

Andrew Leviton as Additional Voices (voice)

David Michie as Additional Voices (voice)

Sumit Naig as Additional Voices (voice)

Juan Pacheco as Additional Voices (voice)

Chrystee Pharris as Additional Voices (voice)

Ben Pronsky as Additional Voices (voice)

Al Rodrigo as Additional Voices (voice)

Stan Sellers as Additional Voices (voice)

Warren Sroka as Additional Voices (voice)

Jasper Johannes Andrews as Additional Voices (voice)

Gredel Berrios Calladine as Additional Voices (voice)

Natalia Castellanos as Additional Voices (voice)

Russell Tyre Francis as Additional Voices (voice)

Deepti Gupta as Additional Voices (voice)

Sohm Kapila as Additional Voices (voice)

Pradnya Kuwadekar as Additional Voices (voice)

Ashley London as Additional Voices (voice)

Christopher Miller as Additional Voices (voice)

Andrea Navedo as Additional Voices (voice)

Lakshmi Patel as Additional Voices (voice)

Jacqueline Pinol as Additional Voices (voice)

Eliyas Qureshi as Additional Voices (voice)

Lashana Rodriguez as Additional Voices (voice)

Jaswant Dev Shrestha as Additional Voices (voice)

Libby Thomas Dickey as Additional Voices (voice)

Jason Linere-White as Additional Voices (voice)

Sitara Attaie as Additional Voices (voice)

Mayuri Bhandari as Additional Voices (voice)

June Christopher as Additional Voices (voice)

Michelle Jubilee Gonzalez as Additional Voices (voice)

Marabina Jaimes as Additional Voices (voice)

Rez Kempton as Additional Voices (voice)

Lex Lang as Additional Voices (voice)

Phil Lord as Additional Voices (voice)

Richard Miro as Additional Voices (voice)

Doug Nicholas as Additional Voices (voice)

Shakira Ja'nai Paye as Additional Voices (voice)

James Pirri as Additional Voices (voice)

Marley Ralph as Additional Voices (voice)

Michelle Ruff as Additional Voices (voice)

Dennis Singletary as Additional Voices (voice)

Amanda Troop as Additional Voices (voice)

Ruth Zalduondo as Additional Voices (voice)

Kimberly Bailey as Additional Voices (voice)

Sanjay Chandani as Additional Voices (voice)

Melanie Duke as Additional Voices (voice)

Jorge R. Gutierrez as Additional Voices (voice)

Miguel Jiron as Additional Voices (voice)

Deepti Kingra-Mickelsen as Additional Voices (voice)

Luisa Leschin as Additional Voices (voice)

Caitlin McKenna as Additional Voices (voice)

Andrew Morgado as Additional Voices (voice)

Arthur Ortiz as Additional Voices (voice)

Eliana A. Perez as Additional Voices (voice)

Juan Pope as Additional Voices (voice)

Mike Rianda as Additional Voices (voice)

Erika Scopelli as Additional Voices (voice)

Narender Sood as Additional Voices (voice)

Cedric L. Williams as Additional Voices (voice)

Kimiko Glenn as Peni Parker (voice) (uncredited)

Peggy Lu as Mrs. Chen (uncredited)