Kate Beckinsale as Selene

Stephen Rea as Dr. Jacob Lane

Michael Ealy as Detective Sebastian

Theo James as David

India Eisley as Eve

Charles Dance as Thomas

Sandrine Holt as Lida

Sanny van Heteren as British newscaster

Jacob Blair as Kolb (Officer)

Robert Lawrenson as Waterfront Cop

Kurt Max Runte as Leader of the Troop

Marvin Duerkholz as Lestroy

Ron Wear as Jack Fletcher

Richard Cetrone as Lycan 1

Dan Payne as Lycan 2

Mark Gibbon as Announcing Guard

Benita Ha as Surgical Nurse

Tyler McClendon as Scientist

Panou as Old City Cop #1

Ian Rozylo as Old City Cop #2

Kristen Holden-Ried as Quint Lane

John Innes as Medical Supervisor

Lee Majdoub as Desk Guard

Catlin Adams as Olivia

Adam Greydon Reid as Med Tech

Christian Tessier as Security Guard #1

Wes Bentley as Antigen Scientist (uncredited)

Scott Speedman as Michael (archive footage) (uncredited)

Joe Censoplano as Cadillac Driver (uncredited)

Preston Cook as Antigen Surgeon (uncredited)