Hugh Jackman as Van Helsing

Kate Beckinsale as Anna Valerious

Richard Roxburgh as Count Vladislaus Dracula

David Wenham as Carl

Shuler Hensley as Frankenstein's Monster

Elena Anaya as Aleera

Will Kemp as Velkan

Kevin J. O'Connor as Igor

Alun Armstrong as Cardinal Jinette

Silvia Colloca as Verona

Josie Maran as Marishka

Tom Fisher as Top Hat

Samuel West as Dr. Victor Frankenstein

Robbie Coltrane as Mr. Hyde (voice)

Stephen Fisher as Dr. Jekyll

Dana Morávková as Barmaid

Zuzana Ďurdinová as Opera Singer

Jaroslav Vízner as Gendarme

Marek Vašut as Villager

Samantha Sommers as Vampire Child

Dorel Mois as Dracula's Ball Performer

Marianna Mois as Dracula's Ball Performer

Laurence Racine as Dracula's Ball Performer

Patrice Wojciechowski as Dracula's Ball Performer

Kacie Borrowman as Dwerger (uncredited)

Martin Klebba as Dwerger (uncredited)

Ryan James as Villager (uncredited)