Frank Welker as Scooby-Doo / Fred Jones (voice)

Grey DeLisle as Daphne Blake (voice)

Matthew Lillard as Shaggy Rogers (voice)

Kate Micucci as Velma Dinkley (voice)

Cassandra Peterson as Elvira, Mistress of the Dark (voice)

Bill Nye as Bill Nye (voice)

Dwight Schultz as Dr. Crane, AKA: Scarecrow (voice)

Kamali Minter as Michelle (voice)

Bumper Robinson as Mike (voice)

Roger Craig Smith as Autopilot / Parent (voice)

Fred Tatasciore as Alpha Jackal-Lantern / Federal Agent Malarkey (voice)

David Herman as Sheriff, AKA: Cutler Toe (voice)