Bob Hoskins as Eddie Valiant

Christopher Lloyd as Judge Doom

Joanna Cassidy as Dolores

Charles Fleischer as Roger Rabbit / Benny The Cab / Greasy / Psycho (voice)

Kathleen Turner as Jessica Rabbit (voice) (uncredited)

Stubby Kaye as Marvin Acme

Alan Tilvern as R.K. Maroon

Richard LeParmentier as Lt. Santino

Lou Hirsch as Baby Herman (voice)

Betsy Brantley as Jessica's Performance Model

Joel Silver as Raoul

Paul Springer as Augie

Richard Ridings as Angelo

Edwin Craig as Arthritic Cowboy

Lindsay Holiday as Soldier

Mike Edmonds as Stretch

Morgan Deare as Editor / Gorilla (voice)

Danny Capri as Kid #1

Christopher Hollosy as Kid #2

Jean-Paul Sipla as Kid #3

Laura Frances as Blonde Starlet

Joel Cutrara as Forensic #1

Billy J. Mitchell as Forensic #2

Eric B. Sindon as Mailman

Ed Herlihy as Newscaster

James O'Connell as Conductor

Eugene Gutierrez as Teddy Valiant

April Winchell as Mrs. Herman (voice)

Mae Questel as Betty Boop (voice)

Mel Blanc as Daffy Duck / Tweety / Bugs Bunny / Sylvester / Porky Pig (voice)

Tony Anselmo as Donald Duck (voice)

Mary T. Radford as Hippo (voice)

Joe Alaskey as Yosemite Sam / Foghorn Leghorn (voice)

David L. Lander as Smart Ass (voice)

Fred Newman as Stupid (voice)

June Foray as Wheezy / Lena Hyena (voice)

Wayne Allwine as Mickey Mouse (voice)

Russi Taylor as Minnie Mouse / Birds (voice)

Les Perkins as Mr. Toad (voice)

Richard Williams as Droopy (voice)

Pat Buttram as Bullet #1 (voice)

Jim Cummings as Bullet #2 (voice)

Jim Gallant as Bullet #3 (voice)

Frank Sinatra as Singing Sword (voice) (archive sound)

Tony Pope as Goofy / Big Bad Wolf (voice)

Peter Westy as Pinocchio (voice)

Cherry Davis as Woody Woodpecker

Jack Angel as Additional Voices (voice) (uncredited)

Jeff Arbaugh as Mime (uncredited)

Nancy Cartwright as Dipped Shoe (voice) (uncredited)

Amy Irving as Jessica Rabbit (singing voice) (uncredited)

Mickie McGowan as Mooing Cow / Flying Witch (voice) (uncredited)

Frank Welker as Dumbo (voice) (uncredited)

Tina Simmons as Ink and Paint Club Patron (uncredited)