Julia Roberts as Hova (voice)

Meryl Streep as Queen (voice)

Nicolas Cage as Zoc (voice)

Paul Giamatti as Stan Beals (voice)

Bruce Campbell as Fugax (voice)

Regina King as Kreela (voice)

Lily Tomlin as Mommo (voice)

Larry Miller as Fred Nickle (voice)

Cheri Oteri as Doreen Nickle (voice)

Zach Tyler Eisen as Lucas Nickle (voice)

Ricardo Montalban as Head of Council (voice)

Myles Jeffrey as Steve (voice)

Creagen Dow as Mullet Boy (voice)

Frank Welker as Spindle / Frog / Caterpillar (voice)

Mark DeCarlo as Fly (voice)

Jake T. Austin as Nicky (voice)

Allison Mack as Tiffany Nickle (voice)

S. Scott Bullock as Glow Worm / Wasp Survivor (voice)

Paul Greenberg as Sleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice)

Rob Paulsen as Beetle (voice)

Nicole Sullivan as Ant #9 (voice)

Don Frye as Soldier Ant (voice)

Larry Cedar as Ant #8 (voice)

Paul Greenberg as Sleeping Ant #1 / Head Lice #1 / Head Lice #2 (voice)

David Kaye as Sleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice)

Alfred Jackson as Slacker Ant (voice)

Ken Mitchroney as Ant #17 / Guard Ant #2 / Wasp #8 (voice)

Denzel Whitaker as Albert (voice)

Casey Masamitsu as Shigeko (voice)

Bryan Fabian as Fernando (voice)

Jake Goldberg as Helmet Boy / Nob / Red Teammate #1 (voice)

Keith Alcorn as Ant #18 / Pupa / Soldier Ant #3 (voice)

John A. Davis as Ant #19 (voice)

Tom Kenny as Drone Ant / Ant #2 / Ant #6 (voice)

Sarah Mensinga as Mother Ant (voice)

Nika Futterman as Ant #1 / Ant #7 (voice)

Scott Holst as Ant #3 (voice)

Susan Silo as Ant #4 (voice)

Paul Rugg as Ant #5 (voice)

Candi Milo as Nurse Ant #3 (voice)

Nissa Alcorn as Billo (voice)

Pat Fraley as Ant Council #1 (voice)

Tyler James Williams as Blue Teammate #1 (voice)

Jared Goldsmith as Blue Teammate #2 / Red Teammate #2 (voice)

Austin Majors as Blue Teammate #3 (voice)

Jaishon Fisher as Blue Teammate #4 (voice)

Aaron Michael Drozin as Blue Teammate #5 (voice)

Max Burkholder as Blue Teammate #6 (voice)

Sam Green as Red Teammate #3 (voice)

Colin Ford as Red Teammate #4 (voice)

Kendall Ryan Sanders as Red Teammate #5 (voice)

Shane Baumel as Red Teammate #6 (voice)

Neil Ross as Wasp #1 / Wasp #5 (voice)

Bob Joles as Wasp #2 (voice)

Wally Wingert as Wasp #3 (voice)

Leon Morenzie as Wasp #4 (voice)

Jonathan David Cook as Wasp #6 (voice)

Sean Donnellan as Soldier Ant #1 (voice)

Tress MacNeille as Old Council Ant (voice)

Richard Green as Wasp Leader (voice)

Clive Robertson as Hova's Wasp (voice)

Vernee Watson-Johnson as Head Nurse (voice)