Kevin James as Otis the Cow (voice)

Courteney Cox as Daisy the Cow (voice)

Sam Elliott as Ben the Cow (voice)

Danny Glover as Miles the Mule (voice)

Wanda Sykes as Bessy the Cow (voice)

Andie MacDowell as Etta the Hen (voice)

David Koechner as Dag the Coyote (voice)

Jeffrey Garcia as Pip the Mouse (voice)

Cam Clarke as Freddy the Ferret (voice)

Rob Paulsen as Peck the Rooster / Gopher / Pizza Twin #1(voice)

Tino Insana as Pig the Pig (voice)

Dom Irrera as Duke the Dog (voice)

S. Scott Bullock as Eddy the Cow (voice)

John DiMaggio as Bud the Cow / Officer O'Hanlon (voice)

Maurice LaMarche as Igg the Cow (voice)

Madeline Lovejoy as Maddy the Chick (voice)

Earthquake as Root the Rooster (voice)

Steve Oedekerk as Snotty Boy / Mr. Beady / Snotty Boy's Father (voice)

Maria Bamford as Mrs. Beady (voice)

Fred Tatasciore as Farmer (voice)

Peter Stenstrom as Skunk (voice)

Jill Talley as Snotty Boy's Mother (voice)

Laraine Newman as Snotty Boy's Friend (voice)

Katie Leigh as Snotty Boy's Friend / Additional Barnyard Voices (voice)

Anna Pistor as Chick (voice)

Thomas Pistor as Chick (voice)

Eliana Bendetson as Chick (voice)

Paul Butcher as Chick (voice)

Khamani Griffin as Chick (voice)

Arlo Levin as Chick (voice)

Liliana Mumy as Chick (voice)

Cydney Neal as Chick (voice)

Cate Ozawa as Chick (voice)

Isaiah Tefilo as Chick (voice)

Georgia Van Newkirk as Chick (voice)

Joni Allen as Singer (voice)

Leigh French as Additional Barnyard Voices (voice)

William Calvert as Additional Barnyard Voices (voice)

Julianne Buescher as Additional Barnyard Voices (voice)

Nicholas Guest as Additional Barnyard Voices (voice)

Audrey Wasilewski as Additional Barnyard Voices (voice)

Phil Proctor as Additional Barnyard Voices (voice)

Archie Hahn as Additional Barnyard Voices (voice)

Chad Einbinder as Additional Barnyard Voices (voice)

Keith Anthony as Additional Barnyard Voices (voice)

Claudette Wells as Additional Barnyard Voices (voice)

Lynne Marie Stewart as Additional Barnyard Voices (voice)

Christie Mellor as Additional Barnyard Voices (voice)

Justin Shenkarow as Additional Barnyard Voices (voice)

Jacqueline Pinol as Additional Barnyard Voices (voice)

Nika Futterman as Additional Barnyard Voices (voice)

Eddie Frierson as Additional Barnyard Voices (voice)