Ben Stiller as Alex (voice)

Chris Rock as Marty / Additional Zebras (voice)

David Schwimmer as Melman (voice)

Jada Pinkett Smith as Gloria (voice)

Sacha Baron Cohen as Julien (voice)

Cedric the Entertainer as Maurice (voice)

Andy Richter as Mort (voice)

Bernie Mac as Zuba (voice)

Alec Baldwin as Makunga (voice)

Sherri Shepherd as Mom (voice)

will.i.am as Moto Moto (voice)

Elisa Gabrielli as Nana (voice)

Tom McGrath as Skipper / Lemur (voice)

Chris Miller as Kowalski (voice)

Christopher Knights as Private (voice)

Conrad Vernon as Mason (voice)

Quinn Dempsey Stiller as Baby Alex (voice)

Declan Swift as Baby Alex (voice)

Fred Tatasciore as Teetsi / Poacher #1 / Elephant (voice)

Eric Darnell as Joe the Witch Doctor / Poacher #2 (voice)

Willow Smith as Baby Glorida (voice)

Thomas Stanley as Baby Marty (voice)

Zachary Gordon as Baby Melman (voice)

Meredith Vieira as Newscaster (voice)

Lesley Stahl as Newscaster (voice)

Al Roker as Newscaster (voice)

David Soren as Lemur (voice)

Phil LaMarr as Guide (voice)

Stephen Kearin as Stephen the Giraffe / Tourist with Video Camera / Rhino (voice)

Dan O'Connor as Tourist with University Shirt / Cape Buffalo (voice)

Edie Mirman as Telephone Recording (voice)

Fergie as Hippo Girlfriend (voice)

Harland Williams as Additional Giraffe (voice)

Danny Jacobs as Tourist with New York T-Shirt (voice)

Bridget Hoffman as Female Tourist (voice)

Terrence Hardy Jr. as Cub (voice)

Conner Rayburn as Little Giraffe (voice)

Holly Dorff as Fish (voice)

David P. Smith as Bobby the Dik Dik (voice)

Lynnanne Zager as Lioness (voice)

Jackie Gonneau as Additional Dik Dik (voice)