Kathryn Beaumont as Alice (voice)

Ed Wynn as Mad Hatter (voice)

Richard Haydn as Caterpillar (voice)

Sterling Holloway as Cheshire Cat (voice)

Jerry Colonna as March Hare (voice)

Verna Felton as Queen of Hearts (voice)

J. Pat O'Malley as Walrus / Carpenter / Dee / Dum (voice)

Bill Thompson as White Rabbit / Dodo (voice)

Heather Angel as Alice's Sister (voice)

Joseph Kearns as Doorknob (voice)

Larry Grey as Bill (voice)

Queenie Leonard as Bird in the Tree (voice)

Dink Trout as King of Hearts (voice)

Doris Lloyd as The Rose (voice)

James MacDonald as Dormouse (voice)

Thurl Ravenscroft as Card Painter (voice)

Bill Lee as Card Painter (voice)

Max Smith as Card Painter (voice)

Bob Hamlin as Card Painter (voice)

Don Barclay as Other Cards (voice)

Lucille Bliss as Sunflower / Tulip (voice) (uncredited)

Pinto Colvig as Flamingo (voice) (uncredited)

Tommy Luske as Young Pansy (voice) (uncredited)

Clarence Nash as Dinah (voice) (uncredited)

Marni Nixon as Singing Flowers (voice) (uncredited)

Norma Zimmer as White Rose (voice) (uncredited)