Kevin Conroy as Bruce Wayne / Batman (voice)

Michael Ansara as Victor Fries / Mr. Freeze (voice)

Loren Lester as Dick Grayson / Robin (voice)

Mary Kay Bergman as Barbara Gordon / Batgirl (voice)

Robert Costanzo as Detective Harvey Bullock (voice)

Mari Devon as Summer Gleeson (voice)

George Dzundza as Dr. Gregory Belson (voice)

Bob Hastings as Commissioner Jim Gordon (voice)

Marilu Henner as Veronica Vreeland (voice)

Dean Jones as Dean Arbagast (voice)

Efrem Zimbalist Jr. as Alfred Pennyworth (voice)

Liane Schirmer as Renée Montoya (voice)

Rahi Azizi as Additional Voices (voice)

Townsend Coleman as Additional Voices (voice)

Brian George as Additional Voices (voice)

Ed Gilbert as Additional Voices (voice)

Carl Lumbly as Additional Voices (voice)

Tress MacNeille as Additional Voices (voice)

Neil Ross as Additional Voices (voice)

Randy Thompson as Additional Voices (voice)

Lauren Tom as Additional Voices (voice)