Steve Carell as Gru (voice)

Jason Segel as Vector (voice)

Miranda Cosgrove as Margo (voice)

Elsie Fisher as Agnes (voice)

Dana Gaier as Edith (voice)

Russell Brand as Dr. Nefario (voice)

Julie Andrews as Gru's Mother (voice)

Will Arnett as Mr. Perkins (voice)

Kristen Wiig as Miss Hattie (voice)

Pierre Coffin as Tim / Bob / Mark / Phil / Stuart (voice)

Chris Renaud as Dave (voice)

Jemaine Clement as Jerry (voice)

Jack McBrayer as Carnival Barker / Tourist Dad (voice)

Danny McBride as Fred McDade (voice)

Mindy Kaling as Tourist Mom (voice)

Rob Huebel as Anchorman (voice)

Ken Daurio as Egyptian Guard (voice)

Ken Jeong as Talk Show Host (voice)

Charles Bright as Additional Voices (voice)

Katie Leigh as Additional Voices (voice)

Ranjani Brow as Additional Voices (voice)

Scott Menville as Additional Voices (voice)

Holly Dorff as Additional Voices (voice)

Edie Mirman as Additional Voices (voice)

Jackie Gonneau as Additional Voices (voice)

Al Rodrigo as Additional Voices (voice)

Wendy Hoffmann as Additional Voices (voice)

Jakob Roston as Additional Voices (voice)

James Kyson as Additional Voices (voice)

John Hans Tester as Additional Voices (voice)

Tony Lee as Additional Voices (voice)

Debi Mae West as Additional Voices (voice)