Steve Carell as Gru (voice)

Kristen Wiig as Lucy (voice)

Benjamin Bratt as Eduardo/El Macho (voice)

Miranda Cosgrove as Margo (voice)

Russell Brand as Dr. Nefario (voice)

Ken Jeong as Floyd (voice)

Steve Coogan as Silas (voice)

Elsie Fisher as Agnes (voice)

Dana Gaier as Edith (voice)

Moisés Arias as Antonio (voice)

Nasim Pedrad as Jillian (voice)

Kristen Schaal as Shannon (voice)

Pierre Coffin as Kevin / Bob / Stuart / Additional Minions / Evil Minions (voice)

Chris Renaud as Additional Minions / Evil Minions / Italian Waiter (voice)

Nickolai Stoilov as Arctic Lab Guards (voice)

Vanessa Bayer as Flight Attendant (voice)

Ava Acres as Additional Voices (voice)

Lori Alan as Additional Voices (voice)

Jack Angel as Additional Voices (voice)

Eva Bella as Additional Voices (voice)

Georgia Cook as Additional Voices (voice)

John Cygan as Additional Voices (voice)

Debi Derryberry as Additional Voices (voice)

Jess Harnell as Additional Voices (voice)

Danny Mann as Additional Voices (voice)

Mona Marshall as Additional Voices (voice)

Mickie McGowan as Additional Voices (voice)

Mason McNulty as Additional Voices (voice)

Alec Medlock as Additional Voices (voice)

Laraine Newman as Additional Voices (voice)

Jan Rabson as Additional Voices (voice)

Andre Robinson as Additional Voices (voice)

Katie Silverman as Additional Voices (voice)

Casey Simpson as Additional Voices (voice)

Claira Nicole Titman as Additional Voices (voice)

Jim Ward as Additional Voices (voice)

April Winchell as Additional Voices (voice)

Joe Matthews as Additional Voices (voice)

Rob Tinkler as Purple Minions (voice) (uncredited)