Danny Trejo as Machete

Michelle Rodriguez as Luz

Jessica Alba as Sartana Rivera

Robert De Niro as Senator John McLaughlin

Steven Seagal as Rogelio Torrez

Cheech Marin as Padre Cortez

Lindsay Lohan as April Booth

Electra Avellan as Nurse Mona

Elise Avellan as Nurse Lisa

Tom Savini as Osiris Amanpour

Don Johnson as Von Jackson

Billy Blair as Von's Henchman

Jeff Fahey as Michael Booth

Shea Whigham as Sniper

Daryl Sabara as Julio

Tito Larriva as Culebra Cruzado

Gilbert Trejo as Jorge

Felix Sabates as Doc Felix

Alicia Rachel Marek as June

Marci Madison as Nurse Fine

Tina Rodriguez as Tristana

Stacy Keach as Doc Franklin

James Parks as Edgar McGraw

Ara Celi as Reporter

Mayra Leal as Chica (Naked Girl)

Tommy Nix as Dishwasher

Alejandro Antonio as Chief

Juan Gabriel Pareja as Rico

Roland Ruiz as Luis

Nina Leon as Machete's Wife

Jason Douglas as Patrolman #1

Mitchell Lance Adams as Patrolman 2

Vic Trevino as Federale Officer

Jim Henry as Guard

Nimród Antal as Booth's Bodyguard #1

Alcides Dias as Booth's Bodyguard #2