Jason O'Mara as Bruce Wayne / Batman (voice)

Stuart Allan as Robin / Damian Wayne (voice)

Thomas Gibson as Slade Wilson / Deathstroke (voice)

Morena Baccarin as Talia al Ghul (voice)

Giancarlo Esposito as Ra's al Ghul (voice)

David McCallum as Alfred Pennyworth (voice)

Xander Berkeley as Dr. Kirk Langstrom (voice)

Sean Maher as Nightwing / Dick Grayson (voice)

Dee Bradley Baker as Man-Bat / Joker / Man-Bat Commando (voice)

Diane Michelle as Francine (voice)

Fred Tatasciore as Killer Croc (voice)

Bruce Thomas as Gordon / Ubu (voice)

Kari Wahlgren as Rebecca (voice)

Andrea Romano as Suit #2 (voice)