Jason O'Mara as Batman / Bruce Wayne (voice)

Yvonne Strahovski as Batwoman / Kathy Kane (voice)

Stuart Allan as Robin / Damian Wayne (voice)

Sean Maher as Nightwing / Dick Grayson (voice)

Morena Baccarin as Talia al Ghul (voice)

Steve Blum as Black Mask / Firefly (voice)

Gaius Charles as Batwing / Luke Fox (voice)

John DiMaggio as Tusk (voice)

Robin Atkin Downes as The Mad Hatter / Jervis Tetch (voice)

James Garrett as Alfred (voice)

Ernie Hudson as Lucius Fox (voice)

Christine Lakin as Reporter (voice)

Vanessa Marshall as Renee (voice)

Richard McGonagle as President (voice)

Matthew Mercer as Hellhound / Chuckie Sol (voice)

Geoff Pierson as Colonel Jacob Kane (voice)

Jason Spisak as The Calculator / Kuttler / Killer Moth (voice)

Bruce Thomas as Commissioner Gordon (voice)

Kari Wahlgren as Ms. Bannister / Kori (voice)

Travis Willingham as The Heretic (voice)