Kevin Conroy as Batman / Bruce Wayne (voice)

Neal McDonough as Deadshot / Floyd Lawton (voice)

Hynden Walch as Harley Quinn / Harleen Quinzel (voice)

Matthew Gray Gubler as The Riddler / Edward Nigma (voice)

Troy Baker as The Joker (voice)

Eric Bauza as Security Guy (voice)

Chris Cox as G.C.P.D. Commissioner James Gordon (voice)

John DiMaggio as King Shark / Nanaue / Bane (voice)

Greg Ellis as Captain Boomerang (voice)

Giancarlo Esposito as Black Spider / Eric Needham (voice)

Jennifer Hale as Killer Frost / Louise Lincoln (voice)

Martin Jarvis as Alfred Pennyworth (voice)

Peter Jessop as Watch Commander (voice)

Christian Lanz as Scarecrow / Dr. Jonathan Crane / Victor Zsasz (voice)

Nolan North as The Penguin / Oswald Cobblepot / KGBeast / Anatoli Knyazev (voice)

Christopher Lee Parson as Stout Guard (voice)

CCH Pounder as Amanda Waller (voice)

Andrea Romano as Woman (voice)

Travis Willingham as Morgue Guy / Penguin Thug (voice)

Mick Wingert as Joker Security Guy (voice)