Bruce Greenwood as Bruce Wayne / Batman (voice)

Jensen Ackles as Jason Todd / Red Hood (voice)

Neil Patrick Harris as Dick Grayson / Nightwing (voice)

Jason Isaacs as Ra's al Ghul (voice)

John DiMaggio as Jack Napier / Joker / Red Hood (voice)

Wade Williams as Black Mask (voice)

Carlos Alazraqui as Chi Chi / Thug #1 / Baton (voice)

Robert Clotworthy as Leon / Thug (voice)

Gary Cole as Bobo / Commissioner Gordon / Shot / Guard (voice)

Brian George as Ra's Assistant (voice)

Kelly Hu as Ms. Li (voice)

Phil LaMarr as Rick / Bulk (voice)

Alexander Martella as Young Robin / Jason Todd (voice)

Vincent Martella as Robin / Jason Todd (voice)

Jim Piddock as Alfred Pennyworth (voice)

Kevin Michael Richardson as Tyler Bramford (voice)

Andrea Romano as Reporter #1 (voice)

Dwight Schultz as Freddie / Drug Dealer (voice)

Fred Tatasciore as Mercenary #1 / Amazo / Guard (voice)

Keri Tombazian as Reporter #2 / Fearsome Hand Leader (voice)

Bruce Timm as The Riddler (voice)

Michael Villani as Reporter #3 (voice)