Bruce Greenwood as Batman / Bruce Wayne (voice)

Jensen Ackles as Red Hood / Jason Todd (voice)

Neil Patrick Harris as Nightwing (voice)

Jason Isaacs as Ra's al Ghul (voice)

John DiMaggio as Joker (voice)

Wade Williams as Black Mask (voice)

Carlos Alazraqui as Chi Chi / Thug #1 / Baton (voice)

Robert Clotworthy as Leon / Thug (voice)

Gary Cole as Bobo / Commissioner Gordon / Shot / Guard (voice)

Brian George as Ra's Assistant (voice)

Kelly Hu as Ms. Li (voice)

Phil LaMarr as Rick / Bulk (voice)

Alexander Martella as Young Robin / Jason Todd (voice)

Vincent Martella as Robin / Jason Todd (voice)

Jim Piddock as Alfred (voice)

Kevin Michael Richardson as Tyler Bramford (voice)

Andrea Romano as Reporter #1 (voice)

Dwight Schultz as Freddie / Drug Dealer (voice)

Fred Tatasciore as Mercenary #1 / Amazo / Guard (voice)

Keri Tombazian as Reporter #2 (voice)

Bruce Timm as The Riddler (voice)

Michael Villani as Reporter #3 (voice)