Sid Haig as Captain Spaulding

Bill Moseley as Otis B. Driftwood

Sheri Moon Zombie as Baby Firefly

Karen Black as Mother Firefly

Erin Daniels as Denise Willis

Chris Hardwick as Jerry Goldsmith

Rainn Wilson as Bill Hudley

Jennifer Jostyn as Mary Knowles

Tom Towles as Lieutenant George Wydell

Walton Goggins as Deputy Steve Naish

Matthew McGrory as Tiny Firefly

Robert Allen Mukes as Rufus 'RJ' Firefly Jr.

Dennis Fimple as Grandpa Hugo Firefly

Jake McKinnon as Rufus 'Earl' Firefly Sr.

Harrison Young as Don Willis

Irwin Keyes as Ravelli

Michael J. Pollard as Stucky

Chad Bannon as Killer Karl

William Bassett as Sheriff Frank Huston

David Reynolds as Richard 'Little Dick' Wick

Joe Dobbs III as Gerry Ober

Judith Drake as Skunk Ape Wife

Gregg Gibbs as Dr. Wolfenstein

Ken Johnson as Skunk Ape Husband

Irvin Mosley Jr. as Lewis Dover

Tom Towles as George Wydell

Walter Phelan as Dr. Satan

Rob Zombie as Dr. Wolfenstein's assistant (uncredited)