Johnny Depp as Rango / Lars (voice)

Isla Fisher as Beans (voice)

Ned Beatty as Mayor (voice)

Bill Nighy as Rattlesnake Jake (voice)

Abigail Breslin as Priscilla (voice)

Alfred Molina as Roadkill (voice)

Stephen Root as Doc / Merrimack / Mr. Snuggles (voice)

Harry Dean Stanton as Balthazar (voice)

Timothy Olyphant as Spirit of the West (voice)

Ray Winstone as Bad Bill (voice)

Ian Abercrombie as Ambrose (voice)

Gil Birmingham as Wounded Bird (voice)

James Ward Byrkit as Waffles / Gordy / Papa Joad / Cousin Murt / Curlie / Knife Attacker / Rodent Kid (voice)

Claudia Black as Angelique (voice)

Blake Clark as Buford (voice)

John Cothran as Elgin (voice)

Patrika Darbo as Delilah / Maybelle (voice)

George DelHoyo as Señor Flan - Mariachi Accordion (voice)

Maile Flanagan as Lucky (voice)

Charles Fleischer as Elbows (voice)

Beth Grant as Bonnie (voice)

Ryan Hurst as Jedidiah (voice)

Vincent Kartheiser as Ezekiel / Lasso Rodent (voice)

Hemky Madera as Chorizo (voice)

Alex Manugian as Spoons (voice)

Mark 'Crash' McCreery as Parsons (voice)

Joe Nuñez as Rock-Eye (voice)

Christopher Lee Parson as Hazel / Kinski / Stump / Clinker / Lenny / Boseefus (voice)

Lew Temple as Furgus / Hitch (voice)

Alanna Ubach as Boo / Cletus / Fresca / Miss Daisy (voice)

Gore Verbinski as Sgt Turley / Crevice / Slim / Lupe - Mariachi Violin (voice)

Kym Whitley as Melonee (voice)

Keith Campbell as Sod Buster (voice)