Micah Abbey as Donatello (voice)

Shamon Brown Jr. as Michelangelo (voice)

Nicolas Cantu as Leonardo (voice)

Brady Noon as Raphael (voice)

Ayo Edebiri as April O'Neil (voice)

Maya Rudolph as Cynthia Utrom (voice)

John Cena as Rocksteady (voice)

Seth Rogen as Bebop (voice)

Rose Byrne as Leatherhead (voice)

Natasia Demetriou as Wingnut (voice)

Giancarlo Esposito as Baxter Stockman (voice)

Jackie Chan as Splinter (voice)

Ice Cube as Superfly (voice)

Paul Rudd as Mondo Gecko (voice)

Post Malone as Ray Fillet (voice)

Hannibal Buress as Genghis Frog (voice)

Jimmy Donaldson as Times Square Guy (voice)

Derek Wilson as Spider (voice)

Lukas Williams as Curious Goon (voice)

Michael Badalucco as Bad Bernie (voice)

Dempsey Pappion as Bald Bronson (voice)

John Capodice as Cab Driver (voice)

Andia Winslow as TV Anchor (voice)

Raechel Wong as News Anchor (voice)

David Faustino as Normal Nate (voice)

Danny Mastrogiorgio as Toupee Tom / Police Officer (voice)

Noel Gibson as Responding Police Officer / Military Advisor (voice)

Myra Owyang as Short Sharon (voice)

Kevin Eastman as Good Human (voice)

Alex Hirsch as Bossy Goon / Scumbug (voice)

Mike Rianda as Paycheck Goon / Chop Shop Boss / Ratatouille Guard (voice)

Bobby Wagner as Man Who Thought a Giant Rat was a Big Cat but was a Rat (voice)

Greg Levitan as Gecko Goon (voice)

Illya Quinteros as Helpful Construction Worker (voice)

Natalie Canizares as Sign My Baby Mom (voice)

Kyler Spears as Apologetic Goon (voice)

Jeff Rowe as Man Who Loves Being Young and Free to Go Places (voice)