Pete Ploszek as Leonardo

Alan Ritchson as Raphael

Jeremy Howard as Donatello

Noel Fisher as Michelangelo

Megan Fox as April O'Neil

Will Arnett as Vern Fenwick

William Fichtner as Eric Sacks

Danny Woodburn as Splinter

Johnny Knoxville as Leonardo (voice)

Tony Shalhoub as Splinter (voice)

Tohoru Masamune as Shredder

Whoopi Goldberg as Bernadette Thompson

Minae Noji as Karai

Abby Elliott as Taylor

Madison Mason as Councilman

Taran Killam as McNaughton

K. Todd Freeman as Dr. Baxter Stockman

Paul Fitzgerald as Dr. O'Neil

Malina Weissman as Young April O'Neil

Venida Evans as Subway Hostage

Mikal Vega as Sacks Bodyguard

Harley Pasternak as Harley Pasternak

Braeson Herold as Reporter

Chris Wylde as Reporter

Chance Kelly as Mr. Rivetti

Rick Chambers as News Anchor

Leyna Nguyen as News Anchor

Derek Mears as Dojo Ninja

Alison Fernández as Rosa Mendez

Gwen Stefani as Self (archive footage) (uncredited)