Milla Jovovich as Alice

Oded Fehr as Carlos Olivera

Ali Larter as Claire Redfield

Iain Glen as Dr. Isaacs

Ashanti as Betty

Christopher Egan as Mikey

Spencer Locke as K-Mart

Matthew Marsden as Slater

Linden Ashby as Chaser

Jason O'Mara as Albert Wesker

Mike Epps as L.J.

Joe Hursley as Otto

James Tumminia as Lab Tech

Madeline Carroll as White Queen

Valorie Hubbard as Ma

Carrie Eklund as Camp Survivor (uncredited)

Brian Steele as Rancid / Tyrant

John Eric Bentley as Umbrella Tech

Kirk B.R. Woller as Scientist

Rick Cramer as Ice Hockey / Corridor Guard

Peter O'Meara as British Envoy

William Abadie as French Envoy

Ramón Franco as Runty

Shane Woodson as Piggy

Geoff Meed as Pock Mark

Rusty Joiner as Eddie

Connor McCoy as Small Boy

Carolina Jiménez as Mirror Woman

Gary A. Hecker as Tyrant Vocal (voice)

Martin Crewes as Chad Kaplan (uncredited)

Richard Epcar as Additional Voices (voice) (uncredited)

Stefen Hayes as Yuri Loginova (uncredited)

Gary Hudson as Umbrella Captain (uncredited)

Ryan McCluskey as Mr. Grey (uncredited)

Carsten Nørgaard as Zombie Steve (uncredited)

César Ámigo Aguilar as Foreign Envoy (uncredited)